Sandwich RestaurantFast Food RestaurantRestaurantTake Out RestaurantCatererSubway
Your local Lake City Subway® Restaurant at 3269 S Hwy 41 brings bold new flavors and classic favorites to guests every day. Enjoy our famous Footlongs, 6" sandwiches, wraps, and salads, ordered quickly in the app or online, through convenient delivery, in-restaurant dining, or curbside pickup. We’re proud to offer a fresh alternative to typical fast food with fresh-cut veggies, tasty proteins, fresh-baked bread, and delicious cookies. We’re also here for your catering needs. All Subway® Restaurants are independently owned and operated by business owners who employ talented Sandwich Artists™.
Explore offerings from Subway on 3269 S Hwy 41 in Lake City, with popular sandwiches, snacks & sides, dessert, and protein bowls available at this location.
Subway 17591-0 Menu en-US
104 items
Sandwiches
Sandwiches
Oven-Roasted Turkey
Oven-roasted Turkey, provolone cheese, lettuce, tomatoes, onions & mayo served on our freshly baked Artisan Italian bread.
Veggie Delite®
Provolone cheese, spinach, cucumbers, peppers, lettuce, tomatoes & onions served on our freshly baked Hearty Multigrain bread.
Grilled Chicken
Tender grilled chicken, Monterey cheddar, lettuce, tomatoes, onions & mayo served on our freshly baked Artisan Italian bread.
Spicy Italian
Spicy Pepperoni, Genoa salami, provolone cheese, jalapenos, lettuce, tomatoes, onions & mayo served on our freshly baked Artisan bread.
Chicken & Bacon Ranch
Tender Rotisserie-style chicken, Monterey cheddar cheese, bacon, Peppercorn Ranch, lettuce, tomatoes & onions served on our freshly baked Artisan bread.
Black Forest Ham
Ham, provolone cheese, lettuce, tomatoes, onions & mayo served on our freshly baked Artisan Italian bread.
Roast Beef
Roast beef, provolone cheese, lettuce, tomatoes, onions & mayo served on our freshly baked Artisan Italian bread.
Meatball Pepperoni
Meatball, pepperoni, marinara sauce, provolone & parmesan cheese served on our freshly baked Italian & Herb bread.
Meatball
Meatballs, marinara sauce, provolone cheese & parmesan cheese served on our freshly baked Artisan Italian bread.
Pizza Sub
Spicy pepperoni, provolone cheese, marinara sauce, green peppers & onions served on our freshly baked Artisan Italian bread.
Oven-roasted turkey, ham, roast beef, American cheese, lettuce, tomatoes, onions & mayo served on our freshly baked Multigrain bread.
Tuna
100% wild caught Tuna salad, provolone cheese, lettuce, tomatoes & onions served on our freshly baked Artisan Italian bread.
Sweet Onion Chicken Teriyaki®
Tender Grilled chicken, American cheese, Sweet Onion Teriyaki sauce, lettuce, tomatoes & onions served on our freshly baked Hearty Multigrain bread.
Chipotle Philly
Juicy Steak, Pepper Jack cheese, Baja Chipotle sauce, peppers & onions served on our freshly baked Artisan bread.
Turkey & Ham
Oven-roasted turkey, ham, American cheese, lettuce, tomatoes, onions & mayo served on our freshly baked Artisan Italian bread.
Cold Cut Combo®
Ham, salami, bologna (all turkey-based), provolone cheese, lettuce, tomatoes, onions & mayo served on our freshly baked Artisan Italian bread.
B.M.T.®
Spicy Pepperoni, Genoa salami, ham, provolone cheese, lettuce, tomatoes, onions & mayo served on our freshly baked Artisan Italian bread.
B.L.T.
Bacon, lettuce, tomatoes & mayo served on our freshly baked Artisan Italian bread.
5 Meat Italian
Pepperoni, salami, turkey, ham, roast beef, provolone cheese, MVP Vinaigrette, lettuce, tomatoes, onions & mayo served on our freshly baked Artisan bread.
All American Club®
Oven-roasted Turkey, ham, American cheese, bacon, lettuce, tomatoes, onions & mayo served on our freshly baked Artisan bread.
Steak Philly
Juicy Steak, American cheese, mayo, peppers & onions served on our freshly baked Artisan bread.
Snacks & Sides
Snacks & Sides
GoGo squeeZ® Apple Apple
GoGo squeeZ® applesauce pouches are a yummy, nutritious snack made from 100% fruit, no sugar added* and no artificial anything. With a resealable cap, GoGo squeeZ® fruit on the go can be enjoyed wherever life takes you.
*Not a Low Calorie Food. Contains 13g of sugar and 70 calories.
Lays® Classic
It all starts with farm-grown potatoes, cooked and seasoned to perfection. So every LAY'S® potato chip is perfectly crispy and full of fresh potato taste. Happiness in Every Bite.®
Lays® Baked Original
SNACK A LITTLE SMARTER™️ with Baked LAY’S®️ Potato Chips. It’s the LAY’S®️ chip you love, just Baked. Perfectly paired with your favorite Subway sandwich.
Doritos® Nacho Cheese
The iconic bold and intense cheesiness of Doritos® Nacho Cheese Flavored Tortilla Chips. Doritos® flavors ignite adventure and inspire action. Are you ready? If so, crunch on.
SunChips® Harvest Cheddar®
The flavor of real cheddar cheese is layered onto a delicious whole grain chip to create this tasty combination.
Miss Vickie’s® Jalapeño
Made with jalapeño seasoning for enough heat to make things deliciously interesting. And every spicy bite is made with no artificial flavors or preservatives.
Dessert
Dessert
Double Chocolate Cookie
Calling all chocolate lovers! Our Double Chocolate Cookie has your name written all over it. It has rich semi-sweet chocolate chips mixed in a chocolate dough, along with white chips, vanilla and other natural flavors. Take one bite and your taste buds will thank you for it.
Chocolate Chip Cookie
One bite of our Chocolate Chip Cookie and you may just find the sudden urge to order 2, 3… maybe 6. It has semi-sweet chocolate chips, vanilla and other natural flavors folded into buttery cookie dough. It’s waiting to be devoured, just don’t say we didn’t warn you!
Raspberry Cheesecake Cookie
Hold the silverware, because our Raspberry Cheesecake Cookie is a hands-on experience. Sweet raspberry flavored pieces, white chips and just the right amount of cream cheese are mixed together to bake the perfect cookie. So go ahead, dive right into this delicious treat and leave the silverware aside.
Oatmeal Raisin Cookie
Our Oatmeal Raisin Cookie is perfectly soft and chewy making it a must have with your order. Rolled oats, molasses, real California Raisins and just the right touch of cinnamon—all mixed and baked for the perfect treat any time of the day, including breakfast, because it’s oatmeal.
White Chip Macadamia Nut Cookie
Our delectable White Chocolate Macadamia Cookie is anything but cookie cutter. That’s because it has a generous amount of tasty ingredients: white chips, macadamia nuts, vanilla and other natural flavors mixed together to prepare a delightfully rich and nutty taste in a cookie. Go ahead and go nuts!
6 Pack Cookie Box
Our 6 Pack Cookie Box is the most delicious out-of-the-box thinking. Pick your favorite flavor and enjoy a half-dozen of fresh-baked cookies. One for you, one for him, one for her and even more to keep sharing…or not! Get the New 6 Pack Cookie Box today and get that sweet treat you’re craving.
Protein Bowls
Protein Bowls
Honey Mustard
Tender Rotisserie-style chicken, Monterey cheddar cheese, pickles, Honey Mustard sauce, cucumbers, green peppers, black olives, tomatoes & onions served on a bed of lettuce & spinach.
Roast Beef
Roast beef, provolone cheese, tomatoes, cucumbers, peppers, olives & onions, served on a bed of lettuce & spinach.
Sweet Onion Chicken Teriyaki®
Tender Grilled chicken, American cheese, Sweet Onion Teriyaki sauce, tomatoes, cucumbers, peppers, olives & onions, served on a bed of lettuce & spinach.
Tuna
100% wild caught Tuna, provolone cheese, tomatoes, cucumbers, peppers, olives & onions, served on a bed of lettuce & spinach.
Steak Philly
Juicy Steak, American cheese, tomatoes, cucumbers, peppers, olives, onions & mayo, served on a bed of lettuce & spinach.
Cold Cut Combo®
Ham, salami, bologna (all turkey-based), provolone cheese, tomatoes, cucumbers, peppers, olives & onions, served on a bed of lettuce & spinach.
5 Meat Italian
Spicy Pepperoni, Genoa salami, oven-roasted turkey, ham, roast beef, provolone cheese, tomatoes, cucumbers, peppers, olives, onions, & mayo, served on a bed of lettuce & spinach.
B.L.T.
Bacon, tomatoes, cucumbers, peppers, olives, & onions, served on a bed of lettuce & spinach.
Turkey & Ham
Oven-roasted Turkey, ham, provolone cheese, tomatoes, cucumbers, peppers, olives, & onions, served on a bed of lettuce & spinach.
B.M.T.®
Spicy Pepperoni, Genoa salami, ham, provolone cheese, tomatoes, cucumbers, peppers, olives, onions & mayo, served on a bed of lettuce & spinach.
Chicken & Bacon Ranch
Tender Rotisserie-style chicken, Monterey cheddar cheese, bacon, Peppercorn Ranch, tomatoes, cucumbers, peppers, olives, & onions served on a bed of lettuce & spinach.
Spicy Italian
Pepperoni, salami, provolone cheese, tomatoes, cucumbers, peppers, olives, onions, jalapenos & mayo, served on a bed of lettuce & spinach.
Meatball
Meatballs, tomatoes, cucumbers, peppers, olives, onions, provolone & parmesan, served on a bed of lettuce & spinach.
Subway Club®
Oven-roasted turkey, ham, roast beef, American cheese, tomatoes, cucumbers, peppers, olives, & onions, served on a bed of lettuce & spinach.
Oven-Roasted Turkey
Oven-roasted Turkey, provolone cheese, tomatoes, cucumbers, peppers, olives & onions, served on a bed of lettuce & spinach.
Grilled Chicken
Tender Grilled chicken, Monterey cheddar cheese, tomatoes, cucumbers, peppers, olives & onions, served on a bed of lettuce & spinach.
All American Club®
Oven-roasted turkey, ham, bacon, American cheese, mayo, tomatoes, cucumbers, peppers, olives, & onions, served on a bed of lettuce & spinach.
Black Forest Ham
Ham, provolone cheese, tomatoes, cucumbers, peppers, olives & onions, served on a bed of lettuce & spinach.
Pizza Sub
Spicy pepperoni, provolone cheese, marinara sauce, green peppers & onions, served on a bed of lettuce & spinach.
Wraps
Wraps
Black Forest Ham
Ham, provolone, lettuce, tomatoes & onions, wrapped in tortilla.
Juicy Steak, egg & melty American cheese served on our freshly baked Artisan bread.
Egg & Cheese Wrap
Egg & melty American cheese, wrapped in tortilla.
Steak, Egg & Cheese Wrap
Juicy Steak, egg & melty American cheese, wrapped in tortilla.
Black Forest Ham, Egg & Cheese
Ham, egg & melty American cheese served on our freshly baked Artisan bread.
Bacon, Egg & Cheese Wrap
Bacon, egg & melty American cheese, wrapped in tortilla.
Egg & Cheese
Egg & melty American cheese served on our freshly baked Artisan bread.
Bacon, Egg & Cheese
Bacon, egg & melty American cheese served on our freshly baked Artisan bread.
Black Forest Ham, Egg & Cheese Wrap
Ham, egg & melty American cheese, wrapped in tortilla.
Salads
Salads
Oven-Roasted Turkey
Oven-roasted Turkey, provolone cheese, tomatoes, cucumbers, peppers, olives & onions, served on a bed lettuce & spinach.
Meatball
Meatballs, tomatoes, cucumbers, peppers, olives, onions, provolones & parmesan cheese, served on a bed lettuce & spinach.
Steak Philly
Juicy Steak, American cheese, tomatoes, cucumbers, peppers, olives, onions & mayo, served on a bed lettuce & spinach.
Chicken & Bacon Ranch
Tender Rotisserie-style chicken, Monterey cheddar cheese, bacon, Peppercorn Ranch, tomatoes, cucumbers, peppers, olives, & onions served on a bed lettuce & spinach.
Veggie Delite®
Tomatoes, provolone cheese, cucumbers, peppers, olives & onions, served on a bed lettuce & spinach.
Tuna
100% wild caught Tuna, provolone cheese, tomatoes, cucumbers, peppers, olives & onions, served on a bed lettuce & spinach.
Roast Beef
Roast beef, provolone cheese, tomatoes, cucumbers, peppers, olives & onions, served on a bed lettuce & spinach.
Spicy Italian
Spicy Pepperoni, Genoa salami, provolone cheese, tomatoes, cucumbers, peppers, olives, onions, jalapenos & mayo, served on a bed lettuce & spinach.
Honey Mustard
Tender Rotisserie-style chicken, Honey Mustard sauce, Monterey cheddar cheese, tomatoes, cucumbers, green peppers, olives, onions & pickles on a bed of lettuce & spinach.
Pizza Sub
Spicy pepperoni, provolone cheese, marinara sauce, green peppers & onions, served on a bed of lettuce & spinach.
Grilled Chicken
Tender grilled chicken, Monterey cheddar cheese, tomatoes, cucumbers, peppers, olives, & onions, served on lettuce & spinach.
Turkey & Ham
Oven-roasted Turkey, ham, provolone cheese, tomatoes, cucumbers, peppers, olives, & onions, served on a bed lettuce & spinach.
Subway Club®
Oven-roasted Turkey, ham, roast beef, American cheese, tomatoes, cucumbers, peppers, olives, onions & mayo served on a bed lettuce & spinach.
Black Forest Ham
Ham, provolone cheese, tomatoes, cucumbers, peppers, olives & onions, served on a bed lettuce & spinach.
B.M.T.®
Spicy Pepperoni, Genoa salami, ham, provolone cheese, tomatoes, cucumbers, peppers, olives, onions & mayo, served on a bed lettuce & spinach.
5 Meat Italian
Spicy Pepperoni, Genoa salami, oven-roasted turkey, ham, roast beef, provolone cheese, tomatoes, cucumbers, peppers, olives, onions, & mayo, served on a bed lettuce & spinach.
Sweet Onion Chicken Teriyaki®
Tender Grilled chicken, American cheese, Sweet Onion Teriyaki sauce, tomatoes, cucumbers, peppers, olives & onions, served on a bed lettuce & spinach.
All American Club®
Oven-roasted Turkey, ham, American cheese, bacon, tomatoes, cucumbers, peppers, olives, onions & mayo served on a bed lettuce & spinach.
Fresh Fit for Kids®
Fresh Fit for Kids®
Veggie Delite® for Kids
Provolone cheese, spinach, cucumbers, peppers, lettuce, tomatoes & onions served on our freshly baked Hearty Multigrain bread plus side & drink.
Black Forest Ham for Kids
Ham, provolone, lettuce, tomatoes, onions & mayo served on our freshly baked Hearty Multigrain bread plus side & drink.
Oven-Roasted Turkey for Kids
Oven-roasted Turkey, provolone cheese, lettuce, tomatoes, onions & mayo served on our freshly baked Hearty Multigrain bread plus side & drink.
Personal Pizza
Personal Pizza
Personal Cheese Pizza
Our pizza dough is covered with mouthwatering marinara sauce then topped with shredded mozzarella cheese. Make it yours by adding your favorite veggies. We make it amazing by baking it to personal perfection.
Drinks
Drinks
Gatorade® Cool Blue
Cool, satisfying taste to quench thirst and energize without caffeine
Honest Kids® Super Fruit Punch
Grape, strawberry, apple, watermelon juices and other ingredients unite to truly pack a punch.
USDA organic certified. Sweetened only with fruit juice. No added sugar. No artificial sweeteners. No high fructose corn syrup. Gluten free. No GMOs.
Celsius® Sparkling Orange
The perfect balance of rich, juicy orange flavor and energy.
Reviews
3.7
45 reviews
5 stars
24
4 stars
5
3 stars
3
2 stars
5
1 star
8
SM
Sam mass
Nov 6, 2025
3.0
I have been here several times and this Subway has gone down hill so much. The service is slow and always seems that they are more concerned with making online pick up orders before the helping the customers standing in line. I ordered a sandwich I have ordered a few times and now all of a sudden I have to pay for cheese on it?? I ordered this sandwich at another location and never have been told that. The last few times I have ordered my bread has been so hard I haven't been able to eat it, I actually had to re-make it on bread at home. This location needs some help.
RM
Ryan Murphy
Apr 15, 2023
1.0
I ordered online went to pickup my sandwich’s, by the time I got back to hotel my Italian BMT only had pepperoni, no ham no salami not toasted. Not cool.
DH
Darlene Head
Aug 2, 2019
1.0
Been there twice won't make the mistake again....1st time they didn't have the carving board turkey and they are scared to give you any toppings..second time lettuce was brown in bin and wahen I requested the spinach I was only given a few leaves for $20 for 2 sandwiches and 3 cookies and to still be hungry is ludicrous.. Go to Burger King at least you get toppings on your sandwich plus fries a drink and a cookie for $6 and you get full and satisfied!
Subway is located at 3269 S Hwy 41, Columbia, Lake City, FL 32025.
What are the operating hours of the Subway restaurant?
Subway is open Monday to Wednesday from 7:00 AM to 9:00 PM, Thursday and Friday from 7:00 AM to 10:00 PM, and Saturday and Sunday from 10:00 AM to 8:00 PM.
Does Subway offer curbside pickup service?
Yes, Subway offers curbside pickup service for your convenience.
Can I order Subway's menu online or through an app?
Yes, you can order Subway quickly and easily through their app or online at their official website.
Does this Subway location offer catering services?
Yes, Subway provides catering services for various events and needs.
What types of meals does this Subway restaurant serve?
This Subway serves breakfast, lunch, and dinner with fresh-baked bread, fresh-cut veggies, and a variety of sandwiches, wraps, and salads.
Is dine-in available at this Subway?
Yes, this Subway location offers dine-in service for customers.
What nearby convenience stores can I visit before or after Subway?
Lake City Convenience Store and Busy Bee are nearby convenience stores where you can shop or pick up essentials along with your Subway visit.
Are there any gas stations close to Subway for a quick fuel-up?
Yes, several Shell gas stations and Busy Bee gas station are located nearby for convenient fueling and shopping needs.
What local salons are near Subway if I want a haircut or beauty services?
Cutting Attractions/Judy Currie, Hair Salon 386, and malindaatsalon386 are nearby salons offering beauty and hair services.
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